Adv Physiol Educ AJP: Endocrinology and Metabolism
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Advan. Physiol. Edu. 28: 29-32, 2004; doi:10.1152/advan.00008.2003
1043-4046/04 $5.00
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ADV PHYSIOL EDUC 28:29-32, 2004
© 2004 American Physiological Society

Teaching in the Laboratory

Simplification of rat intubation on inclined metal plate

Sigrid Kastl1, U. Kotschenreuther1, B. Hille1, J. Schmidt2, H. Gepp1 and W. Hohenberger1

1 Department of Surgery, University of Erlangen-Nurenberg, 91054 Erlangen, Germany 2 Department of Anesthesiology, University of Erlangen-Nurenberg, 91054 Erlangen, Germany

Address for reprint requests and other correspondence: S. Kastl, Dept. of Surgery, Univ. of Erlangen-Nurenberg, Krankenhausstr. 12, 91054 Erlangen, Germany (E-mail: sdkastl{at}aol.com)

Small-animal intubation is often necessary during inhalation anesthesia to allow steady-state conditions for large operations and in vivo experiments in all fields of experimental surgery. In rats, placing an orotracheal tube is technically difficult primarily because of the small size of the subject and the lack of equipment specifically designed for this task. We describe a simple rat intubation technique in which the animal is suspended in dorsal recumbency on an inclined metal plate. The animal, anesthetized with ether, is fixed to a 70°-inclined metal plate in a dorsal position by means of a Mersilene ribbon hooked around the upper incisors. This method of positioning the animal is the most important step in the intubation process and further facilitates the technique already described by other authors. A human otoscope was used as a laryngoscope, intubation was performed using the Seldinger technique, and a 14-gauge intravenous catheter served as an endotracheal tube. This inexpensive technique is quickly learned and can be used in any laboratory. Safe and reliable airway management can thus be achieved, permitting in vivo examinations and operations.

Key words: endotracheal intubation; experimental techniques; laboratory animals




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