Adv Physiol Educ ADInstruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Advan. Physiol. Edu. 26: 256-270, 2002;
1043-4046/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (34)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Murphy, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Murphy, D.
ADV PHYSIOL EDUC 26:256-270, 2002
© 2002 American Physiological Society

APS REFRESHER COURSE REPORT

GENE EXPRESSION STUDIES USING MICROARRAYS: PRINCIPLES, PROBLEMS, AND PROSPECTS

David Murphy

University of Bristol Research Centre for Neuroendocrinology, Bristol Royal Infirmary, Bristol BS2 8HW, England

Abstract

Anumber of mammalian genomes having been sequenced, an important next step is to catalog the expression patterns of all transcription units in health and disease by use of microarrays. Such discovery programs are crucial to our understanding of the gene networks that control developmental, physiological, and pathological processes. However, despite the excitement, the full promise of microarray technology has yet to be realized, as the superficial simplicity of the concept belies considerable problems. Microarray technology is very new; methodologies are still evolving, common standards have yet to be established, and many problems with experimental design and variability have still to be fully understood and overcome. This review will describe the time course of a microarray experiment—RNA isolation from sample, target preparation, hybridization to the microarray probe, data capture, and bioinformatic analysis. For each stage, the advantages and disadvantages of competing techniques are compared, and inherent sources of error are identified and discussed.

Key words: functional genomics; microarray; cDNA; oligonucleotide; bioinformatics




This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
M. G. Bjorklund, C. Natanaelsson, A. E. Karlstrom, Y. Hao, and J. Lundeberg
Microarray analysis using disiloxyl 70mer oligonucleotides
Nucleic Acids Res., March 27, 2008; 36(4): 1334 - 1342.
[Abstract] [Full Text] [PDF]

Home page
 The OncologistHome page
R. S. N. Fehrmann, X.-y. Li, A. G. J. van der Zee, S. de Jong, G. J. te Meerman, E. G. E. de Vries, and A. P. G. Crijns
Profiling Studies in Ovarian Cancer: A Review
Oncologist, August 1, 2007; 12(8): 960 - 966.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. Morrison, J. Hurley, J. Garcia, K. Yoder, A. Katz, D. Roberts, J. Cho, T. Kanigan, S. E. Ilyin, D. Horowitz, et al.
Nanoliter high throughput quantitative PCR
Nucleic Acids Res., October 6, 2006; 34(18): e123 - e123.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Hindmarch, S. Yao, G. Beighton, J. Paton, and D. Murphy
A comprehensive description of the transcriptome of the hypothalamoneurohypophyseal system in euhydrated and dehydrated rats
PNAS, January 31, 2006; 103(5): 1609 - 1614.
[Abstract] [Full Text] [PDF]

Home page
 Health Informatics JournalHome page
M. G. Tyshenko and W. Leiss
Current trends in publicly available genetic databases
Health Informatics Journal, December 1, 2005; 11(4): 295 - 308.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online